Alkaline phosphatase and lactate dehydrogenase enzyme activity in gingival crevicular fluid during orthodontic tooth movements

Abstract

Background: Bone remodeling is a biologic process involving an acute inflammatory response in periodontal
tissues during orthodontic tooth movement. The early response of periodontal tissues to mechanical stress
involves metabolic changes that allow tooth movement.
Aim of the Study: The aim of the present study was to evaluate the changes in alkaline phosphatase (ALP) and
lactate dehydrogenase (LDH) enzyme activity in gingival crevicular fluid (GCF) when forces are applied during
initial alignment (P1) and retraction (P2).
Settings and Design: The study was performed in a hospital‐based population with an experimental design.
Materials and Methods: Seventeen participants (11–21 years) included in the study, all requiring 1st premolar
extractions were undergoing fixed orthodontic treatment. Maxillary canine and mandibular 2nd molar were used
as test tooth (TT) and control tooth (CT) in each patient, respectively. On 14th day after start of each phase,
2 μL of GCF was collected from mesial side of both TT and CT for ALP and LDH activity determination using
spectrophotometer.
Statistical Analysis: Statistical analysis was performed with SPSS (version 13, SPSS Inc., Chicago, USA) package.
The significance of differences in both phases at test or, at control site for LDH and ALP enzyme activity was
evaluated by performing student’s paired t‐test. The significance of differences in between test and control sites
in each phase separately was evaluated by performing student’s unpaired t‐test.
Results: There was a variation in enzyme activity during orthodontic tooth movement. At TT, there was statistically
significant increase in ALP and LDH activity in P2 as compared to P1 with P = 0.005 and P ≤ 0.001, respectively.
At CT, there was no statistically significant difference in ALP and LDH activity in P1 and P2 with P = 0.054 and
P = 0.061, respectively.
Conclusion: This study concluded that ALP and LDH activity could be successfully estimated in the GCF and ALP
and LDH activity in GCF reflects the biologic activity in the periodontium during orthodontic tooth movements.